WebMaterials Required but not Supplied DNA Template: The DNA template must be linear and contain the SP6 RNA Polymerase promoter with the correct orientation in relation to the target sequence to be transcribed. General: Thermocycler or 37°C dry air incubator, microcentrifuge, nuclease-free water, nuclease-free tubes and tips Purification: Phenol, …

MEGAshortscript™ T7 转录试剂盒 - Thermo Fisher

WebThermo Fisher megashort t7 mrna transcription synthesis kit Megashort T7 Mrna Transcription Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebMAXIscript Kits are available for DNA templates containing T7, T3, and SP6 promoters. MEGAshortscript™ T7 Kit P/N 1354 The MEGAshortscript Kit gives high yields of in vitro transcription products in the 20–500 nucleotide range. Many modified nucleotides are efficiently incorporated, but the kit is not recommended for the synthesis of high spe- huawei b535-232 manuale pdf

TranscriptAid T7 高产率转录试剂盒 - Thermo Fisher

Web1 dag geleden · The T7-Cas9 PCR product was purified and used as a template for in vitro transcription using the mMESSAGE mMACHINE T7 ULTRA Kit (Life Technologies). A T7 promoter was fused to the gRNA template by PCR amplification, and the T7-gRNA PCR product was purified and used as a template for in vitro transcription using the … WebMegashortscript T7 Mrna Transcription Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … WebThe MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of … huawei b612 smart hub manual