WebWe next exam- ined the ability of the anti-Ly-49H mAb 3D10 to activate primary NK cells to kill the human FcR Daudi B lym- phoma cell line in the redirected lysis assay (Fig. 5). mAb 3D10 ... Web30 mar. 2009 · The blocking anti-Ly49H mAb (clone 3D10) or an isotype-matched control mAb was added to the indicated cultures at 5 µg/ml. 6 h later, supernatants were harvested and assayed for 51 Cr release. Spontaneous lysis was determined by incubating target cells without effectors. Maximum lysis was determined by incubating target cells in 1% Triton X ...
Ly-49H Antibody, Biotin (13-5886-82) - Thermo Fisher
WebFITC anti-mouse Ly49H Antibody - Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not … Web(B) NK cells in the spleen according to their expression of CXCR3. (C) As described in the legend to panel B but for Ly49H expression. (D) As described in the legend to panel B but for Ly49C/I expression. (E) As described in the legend to panel B but for NKG2A expression. (F) MFI of NKG2D in NK cells in the spleens of the indicated mice. inbound udp
Activating Receptor Signals Drive Receptor Diversity in …
WebThe 3D10 monoclonal antibody specifically binds to mouse Lymphocyte antigen 49H (Ly-49H; also known as Klra8 or Killer cell lectin-like receptor 8). The 3D10 antibody does not crossreact with related molecules such as Ly-49A, C, D or G2. Ly-49H is a type II transmembrane protein and a member of the Ly-49 C-type lectin multigene family of … Web14 mai 2016 · For animals that are productively infected with MCMV, evaluate the numbers and/or percentage of Ly49H + NK cells in the blood or specific organs (e.g., spleen, liver, or lungs) at various time points post-infection. Typically, time points of interest include day 7 (peak of the effector phase), day 14, and day 21 (the contraction phase), and day ... Web22 sept. 2024 · To investigate the role of T and B cells in adaptive NK cell responses against MCMV infection, we compared Ly49H + NK cell expansion in wild-type (WT) mice versus Rag2 −/− mice, which lack adaptive lymphocytes. Whereas WT mice exhibited a robust NK cell response, peaking ∼7 days post-infection (PI), Rag2 −/− mice showed a diminished … inbound turismo