Lineweaver-burk plot是什么
NettetLineweaver Burk plots are a graphical method of analyzing the Michaelis–Menten equation and the enzyme-substrate-inhibitor relationship. The x-axis in the graph is … In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934. The Lineweaver–Burk plot for inhibited enzymes can be compared to no inhibitor to determine how the … Se mer $${\displaystyle [S]}$$: substrate concentration. The independent axis of a Lineweaver-Burk plot is the reciprocal of substrate concentration, $${\displaystyle {\frac {1}{[S]}}}$$. $${\displaystyle V}$$ Se mer The plot provides a very useful graphical method for analysis of the Michaelis–Menten equation, as it is difficult to determine precisely the Vmax of an enzyme-catalysed … Se mer While the Lineweaver-Burk is useful for determining important variables in enzyme kinetics, it is prone to error. The y-axis of the plot takes the reciprocal of the rate of reaction, meaning … Se mer • NIH guide, enzyme assay development and analysis Se mer When used for determining the type of enzyme inhibition, the Lineweaver–Burk plot can distinguish competitive, pure non-competitive and uncompetitive inhibitors. The various modes of inhibition can be compared to the uninhibited reaction. Competitive Inhibition Se mer • Michaelis–Menten kinetics • Eadie–Hofstee diagram • Hanes–Woolf plot Se mer
Lineweaver-burk plot是什么
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NettetLineweaver-Burk-plottet er en simpel linearisering, som tillader grafisk at vurdere, hvorvidt et kinetisk datasæt følger Michaelis-Menten-kinetik . Michaelis-Menten-ligningen kan beskrives som: Hvor V er reaktionshastigheden, Km er Michaelis-konstanten, Vmax er den maksimale reaktionshastighed og [ S] er substratkoncentrationen. NettetKinetic constants for free and immobilized enzyme were also determined by using the Lineweaver-Burk plot.The Km values of the free and immobilized enzymes were …
http://www.biology.arizona.edu/BioMath/tutorials/Linear/LinearFunctionApplication/EnzymeKinetics.html NettetLineweaver Burk plots are another way of representing the data from Michaelis-Menten saturation curves. It originates from the Lineweaver Burk equation, which is derived from the Michaelis-Menten equation below: V 0 = (V max x [S]) / (K M + [S]) The reciprocal of this equation is: 1 / V0 = (KM / Vmax [S]) + (1 / Vmax)
NettetFile:Lineweaver-Burke plot.svg. Size of this PNG preview of this SVG file: 420 × 277 pixels. Other resolutions: 320 × 211 pixels 640 × 422 pixels 1,024 × 675 pixels … NettetThe Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation , in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant. Taking reciprocals of both sides of this equation it becomes as follows:
NettetThe Determination of Km Values from Lineweaver- Burk Plots N C PRICE Department of Biological Science University of Stirling Stirling FK9 4LA, UK Despite its well-known …
NettetHanes–Woolf plot. In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against . It is based on the rearrangement of the Michaelis–Menten equation shown below: human hair grey wigshuman hair grey wigs for black ladiesNettetIn biochimica, il diagramma di Lineweaver-Burk (o diagramma dei doppi reciproci) è una rappresentazione grafica dell' equazione di Lineweaver-Burk della cinetica enzimatica, descritta per la prima volta da Hans Lineweaver e Dean Burk nel 1934. [1] Indice 1 Derivazione 2 Note 3 Altri progetti 4 Collegamenti esterni human hair gray wigs for older women