Webb9 sep. 2024 · Find your tubes from the restriction digest (Part 1). Add 2 µL of Gel green Loading dye into each of the sample tubes. Pipet up and down twice to mix the liquid. Place tubes in a balanced configuration in a MicroCentrifuge and spin for five seconds. WebbHindIII の熱失活条件. 熱処理では不活性化されないため、フェノール処理が必要 ※. 残存活性測定方法： 反応液40 μl 中で、適当な基質DNA 2 μg と、制限酵素30 units とを1 …

HindIII (10 U/µL)

WebbHindIII-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119499. Learn more. We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some … WebbLong fragment 증폭 및 GC-rich template 증폭에 강한 High Fidelity PCR 효소: PrimeSTAR ® GXL DNA Polymerase. 고효율의 Ligation premix: DNA Ligation Kit ＜Mighty Mix＞. TA … kenneth corsello ibm

一种酮还原酶基因及其应用_百度百科

Webb酶切和连接效率：50倍过量的本内切酶消化1小时，>95%被酶切的片段可以被连接并被重新酶切 (recut)。. 活性单位定义：在37℃，50微升反应体系中反应1小时，将1微克的λDNA完全分解的酶量定义为1个活性单位，即1U。. 包装清单：. 产品编号. 产品名称. 包 … HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg via hydrolysis. The cleavage of this sequence between the AA's results in 5' overhangs on th… Webb25 sep. 2002 · 17.5.5 Methylation. The internal HindIII and EcoRI sites in cDNA are protected by AluI and EcoRI methylases, respectively. The cDNA pellet is collected by microcentrifugation, washed once with 70% ethanol, dried in a speed-vac and dissolved in 20 µl of water. To this, add 2.5 µl 10× Alul methylase buffer, 1 µl (S ) … kenneth copeland with pope francis