Web7 de abr. de 2015 · The high lactate concentrations of cultures grown in FortiCHO and CD CHO correlated well with elevated cell-specific lactate production rates of 485 and 421 pg/cell/day, respectively (Table 2). In addition, FortiCHO and CD CHO media triggered the highest specific glucose consumption rates. WebThis has dramatically increased the productivity of mammalian cells in culture but this is accompanied by an increased production and accumulation of lactate in cultures, with the pattern...

The importance of ammonia in mammalian cell culture

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Feeding lactate for CHO cell culture processes: impact on …

Web15 de out. de 1993 · Lactate C3 and acetyl-CoA C2 enrichments were very similar in granule cells, whereas acetyl-CoA C2 enrichment was 60% lower than that of lactate C3 in astrocytes. These results can be explained by the fact that granule cells used almost exclusively the exogenous glucose to fuel the citric acid cycle, whereas astrocytes used … WebAn interesting metabolic response to use as an indirect measure for cell growth in a high glucose medium is the cumulative lactate production of the cells over the culture period. Using lactate measures has the advantage, in an environment with excess amount of glucose, that the ratio between lactate production and glucose consumption is a known … Web5 de jun. de 2012 · Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during … inactives for tnf